Glucotoxicity is mediated by cytoplasmic distribution of RAP1 in pancreatic β-cells

Simple item page
dc.contributorDeręgowska, Anna
dc.contributor.authorDeręgowska, Anna
dc.contributor.authorTomaszek, Natalia
dc.contributor.authorCuch, Patrycja
dc.contributor.authorKozioł, Katarzyna
dc.contributor.authorKaniuka, Olga
dc.contributor.authorSabadashka, Mariya
dc.contributor.authorBandura, Yurii
dc.contributor.authorSybirna, Nataliia
dc.date.accessioned2024-11-12T09:58:56Z
dc.date.available2024-11-12T09:58:56Z
dc.date.issued2024-03-28
dc.descriptionRaw data in a .xlsx: Original data used to generate the presentation of results in figure 1-3.
dc.description.abstractDiabetes mellitus (DM) is a group of chronic metabolic disorders characterized by persistent hyperglycemia. In our study, we analyzed the level and location of RAP1 changes in the development of β-cell dysfunction induced by glucotoxicity. We employed three pancreatic β-cell lines, namely INS-1, 1.2B4, and NIT-1, as well as a streptozotocin-induced diabetes rat model. We demonstrate that after high glucose treatment, RAP1 is increased, probably through induction by AKT, allowing RAP1 to shuttle from the nucleus to the cytoplasm and activate NF-κB signaling. Furthermore, non-enzymatic post-translational modifications of RAP1, such as advanced glycation end products and carbonylation may affect the function of RAP1, such as activation of the NF-κB signaling. Taken together, we showed that RAP1 is a new player in the mechanism of glucotoxicity in pancreatic β-cells.
dc.description.sponsorshipThis work was supported by the National Science Centre of Poland, Miniatura 5, Grant number: 2021/05/X/NZ3/01210
dc.identifier.doi10.1016/j.abb.2024.109982
dc.identifier.urihttps://rdb.ur.edu.pl/handle/item/51
dc.language.isoen
dc.publisherElsevier
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectRAP1
dc.subjectPancreatic β-cells
dc.subjectDiabetes mellitus
dc.subjectHigh glucose treatment
dc.titleGlucotoxicity is mediated by cytoplasmic distribution of RAP1 in pancreatic β-cells
dc.typeraw dataset
Files
Original bundle
Now showing 1 - 1 of 1
Name:
Original dataset used in research paper entitled Glucotoxicity is mediated by cytoplasmic distribution of RAP1 in pancreatic β-cells.xlsx
Size:
689.04 KB
Format:
Microsoft Excel XML
Download
Collections
Instytut Biotechnologii / Institute of Biotechnology