Repozytorium
Danych Badawczych
Uniwersytetu Rzeszowskiego
Communities in RDB
Select a community to browse its collections.
Recent Submissions
Item type: Item , Dataset used in research paper entitled “ITGA1, the alpha 1 subunit of integrin receptor, is a novel marker of drug-resistant senescent melanoma cells in vitro”(Springer Nature, 2025-04-09) Słaby, Julia; Wnuk, Maciej; Błoniarz, Dominika; Stec, Paulina; Szmatoła, Tomasz; Kaznowska, Ewa; Reich, Adam; Moros, María; Lewińska, AnnaChemotherapy-induced senescence may promote drug resistance and treatment failure. Precise detection and elimination of senescent cancer cells is considered as a novel promising anticancer strategy. However, data on senescence-associated skin cancer cell surface markers as potential therapeutic targets are limited. In the present study, we have established two models of drug-induced senescence in vitro using DNA damaging chemotherapeutics, namely etoposide (0.75-5 µM) and cisplatin (1.25-5 µM), and ten skin cancer cell lines, both melanoma (n = 8, A375, G-361, MM370, SH-4, SK-MEL-1, MeWo, MM127, RPMI-7951) and non-melanoma (n = 2, A431, MCC13), to investigate the levels of 97 cell surface markers. Initial gene expression analysis revealed the increasing tendency in the levels of seven transcripts (ITGA1, ITGA3, VAMP3, STX4, ARMCX3, ULBP2, and PLAUR) and five transcripts (ITGA1, ITGA3, STX4, ARMCX3, and PLAUR) in five etoposide and cisplatin-induced senescent melanoma cell lines, respectively, compared to corresponding proliferating cells. Elevated pools of integrin α1 (ITGA1) were confirmed at mRNA and protein levels in eight drug-induced senescent melanoma cell lines. Similar pattern of changes in integrin α1 levels was not observed in drug-induced senescent non-melanoma skin cancer cells. Analysis using clinical melanoma samples also showed that the levels of ITGA1 and ITGA3 were correlated with the presence of melanoma cells in a section. We document that integrin α1 can be considered as a novel marker of drug-induced senescent melanoma cells. Thus, we postulate that new integrin α1-based targeted therapies can be designed and tested against drug-induced senescent melanoma cells.Item type: Item , Comparison of Invasive and Non-Invasive Blood Pressure Measurements During Mechanical Thrombectomy for Acute Ischemic Stroke Under General Anesthesia – A Pilot Study.(2025-07-15) Wiącek, MarcinIntroduction Intra-procedural hypotension during endovascular therapy (EVT) for acute ischemic stroke (AIS) is linked to poorer outcomes. Although continuous invasive blood pressure (IBP) monitoring could help guide timely clinical decisions, it is not routinely implemented during mechanical thrombectomy (MT) under general anesthesia. Objectives This study aimed to assess the agreement between continuous IBP and intermittent non-invasive blood pressure (NIBP) monitoring during EVT and to evaluate whether IBP monitoring impacts procedural timing. Patients and Methods In this prospective observational study, 30 patients undergoing MT for AIS under general anesthesia were enrolled. Blood pressure was simultaneously recorded using radial arterial lines (IBP) and oscillometric cuffs (NIBP, every 5 minutes). Bland–Altman plots were used to assess agreement. Clinically relevant discrepancies and door-to-groin (DTG) times were compared between patients with planned IBP and those without. Results A total of 481 matched BP readings were analyzed. Mean differences between IBP and NIBP for systolic blood pressure (SBP) and mean arterial pressure (MAP) were small (–0.64 mmHg and –0.99 mmHg, respectively), but agreement limits were wide (SBP: –40.6 to 39.4 mmHg; MAP: –28.5 to 26.5 mmHg). Diastolic pressure showed poor agreement (mean bias –7.64 mmHg). Significant SBP differences (≥20 mmHg) occurred in 41.0% of procedure time, and MAP discrepancies (>15 mmHg) in 29.6%. Median DTG times were similar between groups (41 vs. 38 minutes; p = 0.217). Conclusions Intermittent NIBP monitoring demonstrates limited agreement with continuous IBP, particularly under hypotensive conditions, and may fail to detect meaningful hemodynamic shifts. Importantly, the use of IBP does not appear to delay treatment initiation. These results support further investigation in larger studies.Item type: Item , Frequency, preferences, and determinants of energy drink consumption among young polish people after the introduction of the ban on sales to minors(MDPI, 2025) Polak-Szczybyło, EwelinaBackground: In Poland, consumption of energy drink among young people has changed significantly following the introduction of a ban on sales to minors. This reg-ulatory measure was intended to address growing concerns about the health effects of high caffeine consumption among teenagers. The aim of the article was to assess the effectiveness of the ban on the sale of energy drinks to minors and to examine the level of knowledge of young people about the harmfulness of consuming these drinks. Methods: The study was conducted in 2024 in Poland after the introduction of a law that prohibited the consumption of energy drinks among minors. The study group consisted of 999 people aged 15-17. Results: 52% of the respondents declared that they consume ED, and 68% reduced their consumption after the introduction of regulations prohibiting sales to minors. This type of drink is used more frequently by men, and age and frequency were also positively correlated. The factors that most often influenced the choice of ED are price, taste, package size, caffeine content, composition, or recommendations from friends. Conclusions: Although a large percentage of minors still drink energy drinks, new regulations have had an impact on limiting the amount of ED consumed.Item type: Item , Sleep Quality and Body Composition in Active and Inactive Young Adults(2025) Maruszczak, Krystian; Kasperek, Wojciech; Kustra, Konrad; Przysada, Grzegorz; Kochman, MaciejBody composition is influenced by a variety of internal and external factors, with early adulthood being a particularly sensitive period for its fluctuations. This observational study aimed to assess the differences in body composition, anthropometric indices, and sleep quality between active and inactive young adults as well as to explore the relationship between these parameters. A group of one hundred young adults were recruited for the study and assigned to two groups: physically active and physically inactive. Physical activity was assessed using the International Physical Activity Questionnaire (IPAQ-SF), sleep quality was assessed using the Pittsburgh Sleep Quality Index (PSQI), and body composition was measured using the validated InBody 770 analyzer. Participants were classified as physically active if they achieved a "moderate" or "high" activity level according to IPAQ-SF scoring criteria, which includes individuals engaging in at least 600 MET-minutes of physical activity per week. Those categorized as physically inactive scored at the "low" level, not meeting the thresholds for moderate or high activity. Physically active individuals showed significantly more favorable body composition, including higher total body water, muscle mass, and bone mineral content, as well as lower fat mass, BMI, WHR, WHtR, and ABSI values (all p < 0.001). They also had significantly better sleep quality, as indicated by lower Global Sleep Score (GSS) scores (p<0.001). Significant associations were observed between sleep quality and body composition parameters, such as a positive correlation between the GSS and fat mass, as well as negative correlations between GSS and total body water, fat-free mass, and bone mineral content. Additionally, GSS showed positive correlations with both WHR and WHtR (p<0.05). No significant associations were found between the GSS and muscle mass, nor between GSS and ABSI (p>0.05). These findings suggest that regular physical activity is related to more favorable body composition and better sleep quality. Moreover, specific components of body composition, particularly fat mass and indicators of central adiposity, appear to be significantly associated with sleep quality, highlighting a potential link between metabolic health and sleep patterns. However, due to the cross-sectional nature of the study, causality cannot be inferred.Item type: Item , Important role of pregnancy planning for pregnancy outcomes in type 1 diabetes(MDPI, 2025-06-01) Juza, Anna; Dąbrowski, MariuszBackground/Objectives: Pregnancy in women with type 1 diabetes (T1D), compared to general pregnant population, is still associated with an increased number of perinatal complications affecting both fetus and mother. The Great Orchestra of Christmas Charity Foundation (GOCCF) program enables use of continuous subcutaneous insulin infusion (CSII) enhanced by hypo-stop function with real-time continuous glucose monitoring (rtCGM) at the stage of procreation or early pregnancy in T1D patients. The aim of this observational study was to analyze association between pregnancy planning and pregnancy outcomes in patients qualified for the GOCCF program. Methods: 98 women with T1D, aged 21-41 years, initiating CSII + rtCGM system at the stage of planning/early pregnancy or later in case of unplanned pregnancy were quali-fied for the study. We analyzed glycemic control, insulin requirements, pregestational BMI, maternal weight gain, occurrence of preterm birth, congenital malformations and birthweight of newborns. Results: Women who planned pregnancy had significantly better glycemic control before and throughout entire pregnancy and significantly higher proportion of them achieved TIR >70% (58.7% vs. 28.9%, P=0.014) and TAR <25% (65.2% vs. 24.4%, P<0.001). Their glucose variability at the end of pregnancy was significantly lower, 29.4±5.5, vs. 31.9±5.1, P=0.030. They also gave birth later, after mean 37.8±0.9 vs. 36.9±1.8 weeks in non-planning group, P=0.039. Preterm birth oc-curred in 5 women (10,4%) who planned pregnancy and in 15 women (30%) with un-planned gestation, P=0.031. Conclusions: Planning pregnancy in T1D is associated with better glycemic control before conception and throughout entire pregnancy, which is translated into better pregnancy outcomes.