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Item type: Item , Dataset used in research paper entitled “HSP90 inhibition potentiates oxidant-based antimelanoma action of novel thioquercetin derivatives by compromising AhR/CYP1A1 pathway”(Springer Nature, 2026-03-28) Witkowski, Wojciech; Słaby, Julia; Wnuk, Maciej; Stec, Paulina; Piotrowski, Piotr; Żebrowski, Michał; Cybularczyk-Cecotka, Martyna; Deręgowska, Anna; Romanchikova, Nadezhda; Zayakin, Pawel; Linē, Aija; Moros, María; Litwinienko, Grzegorz; Lewińska, AnnaQuercetin, a plant-derived dietary flavonoid, has multifunctional biological activities, including anticancer action; however, its applications may be restricted due to limited bioavailability. Thus, novel synthetic quercetin derivatives (QDs) with improved properties and/or drug combinations should be designed and tested. In the present study, anticancer activity of fourteen newly synthesized QDs was investigated using four cellular models of melanoma, namely A375, MM370, G-361, and SH-4 cells. Thioquercetins (thioQ, thioQ(OAc)4, and thioQ(OAc)5), when used at low micromolar range, induced apoptotic cell death in melanoma cells compared to normal cells. Thioquercetins also reduced the population of spheroid-forming cells and suppressed the growth of A375 cells in 3D spheroid models. Thioquercetin-mediated antimelanoma action was potentiated upon heat shock protein 90 (HSP90) inhibition. Co-treatment with the HSP90 inhibitor 17-DMAG and thioquercetins augmented oxidative stress (increased superoxide production, decreased levels of antioxidant proteins SOD1, and PRDX1-2), and impaired the aryl hydrocarbon receptor (AhR)/cytochrome P450 1A1 (CYP1A1) signaling pathway-based detoxification of thioquercetins by the inhibition of AhR translocation to the nucleus and AhR-mediated stimulation of CYP1A1 expression leading to enhanced cytotoxic effects against melanoma cells. The senolytic activity of thioQ(OAc)4 with four acetylated hydroxy groups against cisplatin-induced senescent melanoma cells was also revealed in selected experimental settings. We suggest that the use of novel thioquercetin-based derivatives along with HSP90 inhibitors should be further validated in vivo and considered for the design of more effective antimelanoma strategies in the future.Item type: Item , Dataset used in research paper entitled “Sulforaphane promotes the removal of doxorubicin-induced senescent epithelial and breast cancer cells and activates AhR/CYP1A1-based adaptive response in vitro”(Elsevier, 2026-03-17) Solarska-Ściuk, Katarzyna; Kapavarapu, Ravikumar; Poliszak, Zuzanna; Wnuk, Maciej; Lewińska, AnnaSulforaphane (SFN), an isothiocyanate derived from cruciferous vegetables, is characterized by antioxidant, anti-inflammatory, and anticancer properties by activating NRF2 pathway, suppressing NFκB pathway, and promoting apoptotic cell death signals and epigenetic changes, respectively. However, SFN-based action against drug-induced senescent cells has never been addressed. In the present study, three breast cancer cell lines with different receptor status, namely triple negative BT-549 and HCC38, and MDA-MB-361 (ER-positive, HER2-positive) along with normal epithelial MCF-10F cells were subjected to 5 μM SFN treatment at both proliferating and non-proliferating (doxorubicin-induced senescence) state. BT-549 proliferating cells were more prone to SFN stimulation than normal ones that was accompanied by oxidative stress-based apoptosis. In contrast, SFN was active in a similar manner against both drug-induced senescent normal and breast cancer cells that resulted in apoptosis-mediated elimination of non-proliferating cells. SFN also stimulated adaptive responses in doxorubicin-induced senescent cells as judged by the activation of aryl hydrocarbon receptor (AhR)/cytochrome P450 1A1 (CYP1A1) pathway and upregulation of heat shock protein 90 (HSP90). In silico analysis with molecular docking also predicted SFN interactions with selected proteins involved in drug detoxification and cell death that warrants further investigation. We postulate that SFN may have senotherapeutic potential, especially against apoptosis resistant chemotherapy-induced senescent normal and breast cancer cells.Item type: Item , Dataset used in research paper entitled “Effect of Fermentation With and Without the Addition of Carrots on the Total Antioxidant Capacity of White and Red Cabbage"(Processes, MDPI, 2025-09-13) Rak, Małgorzata; Bartosz, Grzegorz; Sadowska-Bartosz, IzabelaCabbage is one of the most popular vegetables all over the world, with white cabbage generally being more popular than red cabbage. This study aimed at a comparison of the antioxidant properties of fresh and fermented white and red cabbage. Total phenolic content, the content of anthocyanins and carotenoids, and the Total Antioxidant Capacity (TAC) assayed by ABTS• scavenging, DPPH• scavenging, FRAP, and ORAC of fresh white and red cabbage, fermented white and red cabbage (sauerkraut), and sauerkraut juice were compared. The TAC of fresh and fermented red cabbage, and of red sauerkraut juice (110.3 ± 8.9, 47.4 ± 4.6 and 48.9 ± 5.7 mmol Trolox equivalents/kg, respectively) was significantly higher than the TAC of fresh and fermented white cabbage and white sauerkraut juice (5.1 ± 0.2, 7.9 ± 0.9 and 6.6 ± 0.9 mmol TE/kg, respectively, when assayed by ORAC). The TAC of white sauerkraut and white sauerkraut juice could be elevated by fermentation with 20% of black carrots (to 16.4 ± 1.2 and 10.5 ± 0.8 mmol TE/kg, respectively) but the TAC of red sauerkraut and red sauerkraut juice was diminished by a mixture of either orange or black carrots, which are of lower anthocyanin content than the red cabbage (41.8 ± 3.0 and 29.2 ± 3.1 mmol TE/kg, respectively). These results may justify the promotion of the broad consumption of red cabbage, both fresh and fermented, and encourage the usage of red cabbage as a promising material for functional foods.Item type: Item , Dataset used in research paper entitled “Interaction of Red Cabbage Extract with Exogenous Antioxidants"(International Journal of Molecular Sciences, MDPI, 2025-11-14) Kut, Kacper; Sitarz, Oskar; Kapusta, Ireneusz; Bartosz, Grzegorz; Sadowska-Bartosz, IzabelaInteractions between antioxidants are of interest, mainly for understanding their action in complex biological and food systems. This study aimed to evaluate interactions between the anthocyanin-rich aqueous red cabbage extract and several natural (ascorbic acid, gallic acid, and glutathione) and synthetic (Trolox and TEMPOL) antioxidants as a function of reaction time, concentration, and extract/antioxidant ratio in the ABTS• decolorization and FRAP antioxidant activity assays. The measured interaction type showed dependence on assay type, reaction time, and the extract/antioxidant ratio, but no general dependence on the concentrations of the extract and the antioxidants over a 6-fold concentration range. In the ABTS• decolorization assay, the interactions between the red cabbage extract and exogenous antioxidants were additive (Trolox), weakly antagonistic (ascorbic acid, gallic acid, and glutathione), and definitely antagonistic for TEMPOL. In the FRAP assay, the interactions were additive (ascorbic acid and Trolox), weakly antagonistic (gallic acid and TEMPOL), and definitely synergistic for glutathione. These results suggest the need for a series of assays covering a range of conditions to demonstrate a deviation from additivity in the interactions between antioxidants. The synergy of the interaction of glutathione with the extract in the FRAP assay indicates the possibility of a higher reactivity of this compound in Fe3+ reduction in complex systems than in an assay of the pure compound.Item type: Item , Dataset used in research paper entitled "The Effect of Storage on the Absorption and Fluorescence Spectra of Petal Extracts of Selected Anthocyanin-Containing Flowers"(Processes, MDPI, 2025-06-09) Kut, Kacper; Bartosz, Grzegorz; Sadowska-Bartosz, IzabelaThe biological role of the fluorescence of flowers is a matter of debate. Anthocyanins are a group of compounds that are weakly fluorescent; their fluorescence in flowers has been rarely studied. This study aimed to compare the absorption and fluorescence spectra of anthocyanins extracted from several anthocyanin-containing autumn flowers and examine changes in these spectra during the storage of petals at cold-room and room temperatures and during the storage of dried petals. Petals of red clover Trifolium pratense, pink petunia Petunia × hybrida, Pelargonium horatum, Pelargonium. zonale, Pelargonium. peltatum, red and pink Begonia semperflorens, Buddleja japonica, and purple Chrysanthemum were studied. The results demonstrate that it is possible to distinguish between petals of various flowers based on the absorption spectra of petal extracts and the fluorescence spectra of petal extracts and intact petals. Spectral changes during storage were not always unidirectional and progressive; the most common one was the increase in the intensity of the fluorescence band at 500–560 nm at the excitation wavelength of 460 nm. These results point to the possibility of using fluorescence measurements to identify and estimate the freshness of petal-based material in herbalism, forensic analysis, and the food industry. The measurement of the spectra of whole petals or their fragments by front-face fluorimetry, including common plate readers, may be especially useful due to its simplicity and rapidity.