Surowe dane badawcze do artykułu "Non-targeting siRNA-mediated responses are associated with apoptosis in chemotherapy-induced senescent skin cancer cells"

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dc.contributorLewińska, Anna
dc.contributor.authorBetlej, Gabriela
dc.contributor.authorBłoniarz, Dominika
dc.contributor.authorLewińska, Anna
dc.contributor.authorWnuk, Maciej
dc.date.accessioned2024-03-11T08:09:25Z
dc.date.available2024-03-11T08:09:25Z
dc.date.issued2023-01-05
dc.descriptionExcel file with raw data. Separate sheet for each individual figure in the article (e.g., sheet 1 for figure 1). These raw data were used to prepare bar graphs and box and whisker plots.
dc.description.abstractIt is widely accepted that siRNA transfection can promote some off-target effects in the genome; however, little is known about how the cells can respond to the presence of non-viral dsRNA. In the present study, non-targeting control siRNA (NTC-siRNA) was used to evaluate its effects on the activity of pathogen and host-derived nucleic acid-associated signaling pathways such as cGAS-STING, RIG-I, MDA5 and NF-κB in A431 skin cancer cells and BJ fibroblasts. NTC-siRNA treatment promoted cytotoxicity in cancer cells. Furthermore, NTC-siRNA-treated doxorubicin-induced senescent cancer cells were more prone to apoptotic cell death compared to untreated doxorubicin-induced senescent cancer cells. NTC-siRNA stimulated the levels of NF-κB, APOBECs, ALY, LRP8 and phosphorylated STING that suggested the involvement of selected components of nucleic acid sensing pathways in NTC-siRNA-mediated cell death response in skin cancer cells. NTC-siRNA-mediated apoptosis in cancer cells was not associated with IFN-β-based pro-inflammatory response and TRDMT1-based adaptive response. In contrast, in NTC-siRNA-treated fibroblasts, an increase in the levels of RIG-I and IFN-β was not accompanied by affected cell viability. We propose that the use of NTC-siRNA in genetic engineering may provoke a number of unexpected effects that should be carefully monitored. In our experimental settings, NTC-siRNA promoted the elimination of doxorubicin-induced senescent cancer cells that may have implications in skin cancer therapies.
dc.description.sponsorshipThe experiments involving A431 skin cancer cells were supported by National Science Centre (Poland) OPUS grant UMO-2017/25/B/NZ2/01983 for M.W., whereas the experiments using BJ human fibroblasts were funded by National Science Centre (Poland) OPUS grant UMO-2021/43/B/NZ2/02210 for A.L.
dc.identifier.citationBetlej G, Błoniarz D, Lewińska A, Wnuk M. Non-targeting siRNA-mediated responses are associated with apoptosis in chemotherapy-induced senescent skin cancer cells. Chem Biol Interact. 2023 Jan 5;369:110254.
dc.identifier.doi10.1016/j.cbi.2022.110254
dc.identifier.urihttps://rdb.ur.edu.pl/handle/item/37
dc.language.isoen
dc.publisherChemico-Biological Interactions
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectApoptosis
dc.subjectDoxorubicin
dc.subjectNon-targeting control siRNA
dc.subjectNucleic acid sensing
dc.subjectSenescence
dc.subjectSkin cancer
dc.titleSurowe dane badawcze do artykułu "Non-targeting siRNA-mediated responses are associated with apoptosis in chemotherapy-induced senescent skin cancer cells"
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