Orginal dataset used to generate the presentation of results in reserach paper entitled "Carbon-Coated Iron Oxide Nanoparticles Promote Reductive Stress-Mediated Cytotoxic Autophagy in Drug-Induced Senescent Breast Cancer Cells"
Date
2024-03-14
Journal Title
Journal ISSN
Volume Title
Depositor
Publisher
ACS Applied Materials & Interfaces
Abstract
The surface modification of magnetite nanoparticles (Fe3O4 NPs) is a promising approach to obtaining biocompatible and multifunctional nanoplatforms with numerous applications in biomedicine, for example, to fight cancer. However, little is known about the effects of Fe3O4 NP-associated reductive stress against cancer cells, especially against chemotherapy-induced drug-resistant senescent cancer cells. In the present study, Fe3O4 NPs in situ coated by dextran (Fe3O4@Dex) and glucosamine-based amorphous carbon coating (Fe3O4@aC) with potent reductive activity were characterized and tested against drug-induced senescent breast cancer cells (Hs 578T, BT-20, MDA-MB-468, and MDA-MB-175-VII cells). Fe3O4@aC caused a decrease in reactive oxygen species (ROS) production and an increase in the levels of antioxidant proteins FOXO3a, SOD1, and GPX4 that was accompanied by elevated levels of cell cycle inhibitors (p21, p27, and p57), proinflammatory (NFκB, IL-6, and IL-8) and autophagic (BECN1, LC3B) markers, nucleolar stress, and subsequent apoptotic cell death in etoposide-stimulated senescent breast cancer cells. Fe3O4@aC also promoted reductive stress-mediated cytotoxicity in nonsenescent breast cancer cells. We postulate that Fe3O4 NPs, in addition to their well-established hyperthermia and oxidative stress-mediated anticancer effects, can also be considered, if modified using amorphous carbon coating with reductive activity, as stimulators of reductive stress and cytotoxic effects in both senescent and nonsenescent breast cancer cells with different gene mutation statuses.
Description
The data presented in this study are available in the Supporting Information in https://pubs.acs.org/doi/10.1021/acsami.3c17418 (Physicochemical characterization of Fe3O4@Dex and Fe3O4@aC NPs; TGA curves; results of DLS measurements; stability measurements; effects of Fe3O4@Dex and Fe3O4@aC on metabolic activity in breast cancer cells; effects of Fe3O4@Dex and Fe3O4@aC on metabolic activity and apoptosis induction in epithelial cell line MCF 10F and BJ fibroblasts, description: scavenging activity tests in cell-free system; results of the scavenging tests of Fe3O4@aC NPs; and comparison of FTIR spectra of Fe3O4@aC and Fe3O4@Dex with spectra of samples after the reaction with ROS (docx) List of the mutated gene set in eight breast cancer cell lines (genes related to cell cycle, stress, and autophagy) (xlsx)).
Dataset 1: Additional original data used to generate the presentation of results in figure 1, SI1, SI7-8.
Dataset 2: Original data used to generate the presentation of results in figure 2-10, SI5 and 6B.
Dataset 3: Original genetic data used to generate the presentation of results in figure 6B, 7C, 8B and Table S1-3
Keywords
Fe3O4 nanoparticles, breast cancer, carbon coating, chemotherapy-induced senescence, cytotoxicity
Related publications
Lewińska A, Radoń A, Gil K, Błoniarz D, Ciuraszkiewicz A, Kubacki J, Kądziołka-Gaweł M, Łukowiec D, Gębara P, Krogul-Sobczak A, Piotrowski P, Fijałkowska O, Wybraniec S, Szmatoła T, Kolano-Burian A, Wnuk M. Carbon-Coated Iron Oxide Nanoparticles Promote Reductive Stress-Mediated Cytotoxic Autophagy in Drug-Induced Senescent Breast Cancer Cells. ACS Appl Mater Interfaces. 2024 Mar 27;16(12):15457-15478.
The license associated with this item
Attribution 4.0 International
Research funding institutions
This work was supported by the National Science Centre (NCN, Poland) grant OPUS 22 No. 2021/43/B/NZ7/02129
Type
raw dataset