Surowe dane badawcze do artykułu: Efficient production of bacterial antibiotics aminoriboflavin and roseoflavin in eukaryotic microorganisms, yeasts
Date
2023-07-20
Journal Title
Journal ISSN
Volume Title
Depositor
Publisher
BMC, Spinger Nature
Abstract
Background
Actinomycetes Streptomyces davaonensis and Streptomyces cinnabarinus synthesize a promising broad-spectrum antibiotic roseoflavin, with its synthesis starting from flavin mononucleotide and proceeding through an immediate precursor, aminoriboflavin, that also has antibiotic properties. Roseoflavin accumulation by the natural producers is rather low, whereas aminoriboflavin accumulation is negligible. Yeasts have many advantages as biotechnological producers relative to bacteria, however, no recombinant producers of bacterial antibiotics in yeasts are known.
Results
Roseoflavin biosynthesis genes have been expressed in riboflavin- or FMN-overproducing yeast strains of Candida famata and Komagataella phaffii. Both these strains accumulated aminoriboflavin, whereas only the latter produced roseoflavin. Aminoriboflavin isolated from the culture liquid of C. famata strain inhibited the growth of Staphylococcus aureus (including MRSA) and Listeria monocytogenes. Maximal accumulation of aminoriboflavin in shake-flasks reached 1.5 mg L− 1 (C. famata), and that of roseoflavin was 5 mg L− 1 (K. phaffii). Accumulation of aminoriboflavin and roseoflavin by K. phaffii recombinant strain in a bioreactor reached 22 and 130 mg L− 1, respectively. For comparison, recombinant strains of the native bacterial producer S. davaonensis accumulated near one-order less of roseoflavin while no recombinant producers of aminoriboflavin was reported at all.
Conclusions
Yeast recombinant producers of bacterial antibiotics aminoriboflavin and roseoflavin were constructed and evaluated.
Description
.rar file with raw data, including codon-optimized gene sequences, plasmid sequences and data obtained from HPLC and MS analyses, etc. being the basis for a scientific publication to which they are associated.
Keywords
Yeast, Antibiotics, Metabolic engineering
Related publications
Dmytruk, K. V., Ruchala, J., Fayura, L. R., Chrzanowski, G., Dmytruk, O. V., Tsyrulnyk, A. O., Andreieva, Y. A., Fedorovych, D. V., Motyka, O. I., Mattanovich, D., Marx, H., & Sibirny, A. A. (2023). Efficient production of bacterial antibiotics aminoriboflavin and roseoflavin in eukaryotic microorganisms, yeasts. Microbial cell factories, 22(1), 132. https://doi.org/10.1186/s12934-023-02129-8
The license associated with this item
Attribution 4.0 International
Research funding institutions
This study was supported by Polish National Science Center, grant 2021/41/B/NZ1/01224, National Research Foundation of Ukraine, grant 2020.01/0090, The Centre for International Cooperation & Mobility (ICM) of the Austrian Agency for International Cooperation in Education and Research (OeAD-GmbH) supported this research by grants PL 09/2016 and UA 05/2019.
Type
raw dataset