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Item type: Pozycja , Dataset used in research paper entitled “Fabrication of Electrospun PCL/PEO Microfibers with Fe3O4 Nanoparticles for Magnetic Hyperthermia: Immunocompatibility Assessment Using CD14+ Monocytes, CD4+ and CD8+ T Cells, and CD56+ NK Cells In Vitro”(ACS Publications, 2026-04-15) Radoń, Adrian; Deręgowska, Anna; Ciuraszkiewicz, Agnieszka; Hawełek, Łukasz; Spałek, Hanna; Warski, Tymon; Hudecki, Andrzej; Łukowiec, Dariusz; Piotrowski, Piotr; Krogul-Sobczak, Agnieszka; Rost-Roszkowska, Magdalena; Chajec, Łukasz; Pieszko, Weronika; Bukała, Julia; Rzeszutek, Julia; Wnuk, Maciej; Lewińska, AnnaMagnetite nanoparticles (Fe3O4 NPs), due to their unique physicochemical properties, are considered as promising nanomaterials for multiple biomedical applications. However, the development of novel strategies for surface modification and coating of Fe3O4 NPs is needed to fabricate Fe3O4 NPs with improved biocompatibility. In the present study, two polymers, namely, poly(ε-caprolactone) (PCL) and poly(ethylene oxide) (PEO), were applied to produce PCL/PEO microfibers containing Fe3O4 NPs (PCL/PEO/Fe3O4 MFs) using the electrospinning method. Their physicochemical properties, especially magnetically induced hyperthermia effects, were compared to Fe3O4 NPs. The biocompatibility and immunocompatibility of PCL/PEO/Fe3O4 MFs were then tested using four types of human immune cells, namely, CD14+ monocytes, CD4+ helper, CD8+ cytotoxic T cells, and CD56+ NK cells. Monocytes were the most sensitive to PCL/PEO/Fe3O4 MFs as judged by the induction of cell death (apoptosis and necrosis) and micronuclei production, whereas other immune cells were less or not affected by the stimulation with PCL/PEO/Fe3O4 MFs. PCL/PEO/Fe3O4 MFs also did not lower the viability of normal human fibroblasts. Furthermore, a mild immunogenic response was revealed in PCL/PEO/Fe3O4 MF-treated helper T cells based on the analysis of transcriptional activity of 92 genes involved in the NFκB pathway. Observed elevated mRNA levels of NFKB2, TNF, TNFAIP3, TRAF1, and TBK1 may have context-dependent immunomodulatory effects in PCL/PEO/Fe3O4 MF-stimulated helper T cells that should be taken into account while designing novel drug-delivery systems based on PCL/PEO and Fe3O4 NPs.Item type: Pozycja , Miniatura o nr 2023/07/X/HS3/01581(2026) Dębiec, MaciejMetadane pozyskane w trakcie realizacji projektu Miniatura nr 2023/07/X/HS3/01581. Projekt pt. „Neolit Tracji Egejskiej”.Item type: Pozycja , Dataset used in research paper entitled “HSP90 inhibition potentiates oxidant-based antimelanoma action of novel thioquercetin derivatives by compromising AhR/CYP1A1 pathway”(Springer Nature, 2026-03-28) Witkowski, Wojciech; Słaby, Julia; Wnuk, Maciej; Stec, Paulina; Piotrowski, Piotr; Żebrowski, Michał; Cybularczyk-Cecotka, Martyna; Deręgowska, Anna; Romanchikova, Nadezhda; Zayakin, Pawel; Linē, Aija; Moros, María; Litwinienko, Grzegorz; Lewińska, AnnaQuercetin, a plant-derived dietary flavonoid, has multifunctional biological activities, including anticancer action; however, its applications may be restricted due to limited bioavailability. Thus, novel synthetic quercetin derivatives (QDs) with improved properties and/or drug combinations should be designed and tested. In the present study, anticancer activity of fourteen newly synthesized QDs was investigated using four cellular models of melanoma, namely A375, MM370, G-361, and SH-4 cells. Thioquercetins (thioQ, thioQ(OAc)4, and thioQ(OAc)5), when used at low micromolar range, induced apoptotic cell death in melanoma cells compared to normal cells. Thioquercetins also reduced the population of spheroid-forming cells and suppressed the growth of A375 cells in 3D spheroid models. Thioquercetin-mediated antimelanoma action was potentiated upon heat shock protein 90 (HSP90) inhibition. Co-treatment with the HSP90 inhibitor 17-DMAG and thioquercetins augmented oxidative stress (increased superoxide production, decreased levels of antioxidant proteins SOD1, and PRDX1-2), and impaired the aryl hydrocarbon receptor (AhR)/cytochrome P450 1A1 (CYP1A1) signaling pathway-based detoxification of thioquercetins by the inhibition of AhR translocation to the nucleus and AhR-mediated stimulation of CYP1A1 expression leading to enhanced cytotoxic effects against melanoma cells. The senolytic activity of thioQ(OAc)4 with four acetylated hydroxy groups against cisplatin-induced senescent melanoma cells was also revealed in selected experimental settings. We suggest that the use of novel thioquercetin-based derivatives along with HSP90 inhibitors should be further validated in vivo and considered for the design of more effective antimelanoma strategies in the future.Item type: Pozycja , Dataset used in research paper entitled “Sulforaphane promotes the removal of doxorubicin-induced senescent epithelial and breast cancer cells and activates AhR/CYP1A1-based adaptive response in vitro”(Elsevier, 2026-03-17) Solarska-Ściuk, Katarzyna; Kapavarapu, Ravikumar; Poliszak, Zuzanna; Wnuk, Maciej; Lewińska, AnnaSulforaphane (SFN), an isothiocyanate derived from cruciferous vegetables, is characterized by antioxidant, anti-inflammatory, and anticancer properties by activating NRF2 pathway, suppressing NFκB pathway, and promoting apoptotic cell death signals and epigenetic changes, respectively. However, SFN-based action against drug-induced senescent cells has never been addressed. In the present study, three breast cancer cell lines with different receptor status, namely triple negative BT-549 and HCC38, and MDA-MB-361 (ER-positive, HER2-positive) along with normal epithelial MCF-10F cells were subjected to 5 μM SFN treatment at both proliferating and non-proliferating (doxorubicin-induced senescence) state. BT-549 proliferating cells were more prone to SFN stimulation than normal ones that was accompanied by oxidative stress-based apoptosis. In contrast, SFN was active in a similar manner against both drug-induced senescent normal and breast cancer cells that resulted in apoptosis-mediated elimination of non-proliferating cells. SFN also stimulated adaptive responses in doxorubicin-induced senescent cells as judged by the activation of aryl hydrocarbon receptor (AhR)/cytochrome P450 1A1 (CYP1A1) pathway and upregulation of heat shock protein 90 (HSP90). In silico analysis with molecular docking also predicted SFN interactions with selected proteins involved in drug detoxification and cell death that warrants further investigation. We postulate that SFN may have senotherapeutic potential, especially against apoptosis resistant chemotherapy-induced senescent normal and breast cancer cells.Item type: Pozycja , Dataset used in research paper entitled “Effect of Fermentation With and Without the Addition of Carrots on the Total Antioxidant Capacity of White and Red Cabbage"(Processes, MDPI, 2025-09-13) Rak, Małgorzata; Bartosz, Grzegorz; Sadowska-Bartosz, IzabelaCabbage is one of the most popular vegetables all over the world, with white cabbage generally being more popular than red cabbage. This study aimed at a comparison of the antioxidant properties of fresh and fermented white and red cabbage. Total phenolic content, the content of anthocyanins and carotenoids, and the Total Antioxidant Capacity (TAC) assayed by ABTS• scavenging, DPPH• scavenging, FRAP, and ORAC of fresh white and red cabbage, fermented white and red cabbage (sauerkraut), and sauerkraut juice were compared. The TAC of fresh and fermented red cabbage, and of red sauerkraut juice (110.3 ± 8.9, 47.4 ± 4.6 and 48.9 ± 5.7 mmol Trolox equivalents/kg, respectively) was significantly higher than the TAC of fresh and fermented white cabbage and white sauerkraut juice (5.1 ± 0.2, 7.9 ± 0.9 and 6.6 ± 0.9 mmol TE/kg, respectively, when assayed by ORAC). The TAC of white sauerkraut and white sauerkraut juice could be elevated by fermentation with 20% of black carrots (to 16.4 ± 1.2 and 10.5 ± 0.8 mmol TE/kg, respectively) but the TAC of red sauerkraut and red sauerkraut juice was diminished by a mixture of either orange or black carrots, which are of lower anthocyanin content than the red cabbage (41.8 ± 3.0 and 29.2 ± 3.1 mmol TE/kg, respectively). These results may justify the promotion of the broad consumption of red cabbage, both fresh and fermented, and encourage the usage of red cabbage as a promising material for functional foods.