Repozytorium
Danych Badawczych
Uniwersytetu Rzeszowskiego
Communities in RDB
Select a community to browse its collections.
Recent Submissions
Item type: Item , Dataset used in research paper entitled “TRDMT1 methyltransferase gene knockout attenuates STING-based cell death signaling during self-extracellular RNA-mediated response in drug-induced senescent osteosarcoma cells”(Springer Nature, 2025-08-13) Betlej, Gabriela; Deręgowska, Anna; Wnuk, Maciej; Błoniarz, Dominika; Szmatoła, Tomasz; Klimczak, Katarzyna; Adamczyk-Grochala, Jagoda; Świętoń, Julia; Lewińska, AnnaUnder stress conditions, endogenous biomolecules such as nucleic acids or proteins can be released from damaged cells and considered as damage-associated molecular patterns (DAMPs) activating innate immune system and context-dependent responses. In the present study, self-extracellular RNA was obtained from dying (RNA D) and senescent (RNA S) cellular models of osteosarcoma (OS), characterized by NGS, and tested against proliferating and non-proliferating (etoposide-indued senescent) OS cells (U-2 OS, SaOS-2, MG-63, 143B). RNA D and RNA S induced apoptosis, nitro-oxidative stress, nucleic acid sensing pathways and cytokine production, and RNA m5C methyltransferase-based responses (TRDMT1 and NSUN2) in proliferating OS cells. In drug-induced senescent OS cells, TRDMT1 gene knockout (KO) prevented STING activation, related proinflammatory response, and cell death. Furthermore, IFN-β binding RNA partners were identified, namely NSUN2, NSUN5, NSUN6, CDKN1A, MYC, and RAD51 transcripts and these interactions were compromised in TRDMT1 KO cells and upon RNA D and RNA S treatment. TRDMT1 KO also resulted in replication stress in OS cells that was potentiated by RNA D and RNA S stimulation and associated with elevated levels of APOBEC3A and APOBEC3G, members of the cytidine deaminase protein family. In conclusion, we showed that TRDMT1 KO restricted STING-based immune and cell death response to RNA D and RNA S in non-proliferating drug resistant OS cells that might have potential therapeutic implications.Item type: Item , Acorn dispersal complete database without(2026-01) Bobiec, Andrzej; Kaniuczak, DariuszComplete database table containing the information on 670 records of radio-tracked militransmitters ("Database" sheet), collected in 2022, 2023, 2024, and 2025 seasons. The attribute definitions are provided in the "Field definitions" sheet.Item type: Item , Effect of foliar application of microelements on chlorophyll content, canopy architecture indicators, and physiological parameters of Hordeum vulgare L. plants(PeerJ Publishing, 2025-09-18) Stadnik, Barbara; Tobiasz-Salach, Renata; Migut, DagmaraBackground The availability of nutrients determines the proper development and yield of plants. Microelements play an important role primarily as cofactors of important enzymes in the metabolic and physiological processes of plants. Barley (Hordeum vulgare L.) is one of the most popular cereals in the world. It is used mainly as animal feed and as a raw material for the brewing industry. Methods A three-year field experiment was conducted to determine the effect of foliar spraying of plants with single-component fertilizers containing copper, manganese, molybdenum, and zinc on the physiological parameters of barley plants. In the experiment, three malting barley cultivars were tested: Baryłka, KWS Irina, RGT Planet. During the vegetation period of the plants after foliar fertilization, canopy architecture indices, chlorophyll content in leaves, and gas exchange and chlorophyll fluorescence parameters were measured. Results The foliar application of each of the microelements tested caused a significant increase in the chlorophyll content in the leaves and canopy architecture parameters (LAI and MTA) compared to the control. Furthermore, a significant increase in gas exchange parameters was also observed: intercellular CO2 concentration (Ci), transpiration rate (E), stomatal conductance (gs), net photosynthetic rate (PN) and chlorophyll fluorescence parameters, maximum quantum yield of PSII photochemistry (Fv/Fm), fraction of active PSII reaction centers (RC/ABS), potential photochemical efficiency (Fv/F0), and performance index (PI). The highest results of physiological parameters such as CCI, Ci, E, Fv/Fm, Fv/F0 and PI were recorded after the application of zinc fertilizer. LAI and MTA were highest in plots with plants sprayed with molybdenum-based fertilizer. The foliar application of Cu caused the greatest increase in Ci and PN. The experiment showed significant genotypic differences. The RGT Planet cultivar had a higher chlorophyll content, E and PN parameters among the tested cultivars. The KWS Irina cultivar was characterized by the highest values of canopy architecture parameters and significantly lower values of CCI, Fv/Fm and Fv/F0 compared to the Baryłka and RGT Planet.Item type: Item , Impact of Foliar Application of Copper, Manganese, Molybdenum, and Zinc on the Chemical Composition and Malting Quality of Barley Cultivars(MDPI (Multidisciplinary Digital Publishing Institute), 2025-11-20) Stadnik, Barbara; Tobiasz-Salach, Renata; Migut, DagmaraThe aim of this study was to evaluate the effect of foliar application of selected micro-nutrients on the chemical composition and malting quality of spring barley (Hordeum vulgare L.). The scientific literature lacks in-depth studies that assess the effect of foliar application of micronutrients on barley malting quality. Most studies (especially under field conditions) focus on nitrogen fertilization rather than individual micronutrients. Three brewing-type barley cultivars (Baryłka, KWS Irina, and RGT Planet) were evaluated under foliar micronutrient fertilization (Cu, Mn, Mo, Zn). Fertilizers were applied at doses of 2 L ha−1 for Cu, Mn, and Zn and 1 L ha−1 for Mo. The experiment examined the hectoliter mass, theoretical extractability, contents of selected micro- and macronutrients, and the protein, fat, fiber, and ash contents of the grain. Furthermore, the following characteristics of barley malt were determined, i.e., moisture, protein, extractivity, Kolbach index, and diastatic power. The results showed significant variability in grain and malt quality depending on the cultivar and year. The Baryłka cultivar was characterized by the highest grain density (66.3 kg hL−1) and protein content (10.9% d.m.), while RGT Planet had the highest extractivity and the most favorable malting profile. Foliar supplementation had a slightly positive effect on the average content of trace elements in barley. Mn application increased grain Ca content by 5.6% compared with the control, while foliar Zn fertilization resulted in the highest zinc concentration (a 24.7% increase). No significant effect of fertilization on malt quality was observed, but a significant interaction of experimental factors in extractivity, Kolbach index, and diastatic power was noted. The obtained results indicate that a single foliar application of microelements affects the contents of minerals and protein in the grain, but it does not lead to a significant improvement in malting parameters. This suggests the need for further research on dosage, application date, and interactions between the cultivar and environmental conditions.Item type: Item , Wizualizacja SEM(Uniwersytet Rzeszowski, 2025-12-16) Kus-Liśkiewicz, Małgorzata; Płoch, DariuszAnaliza i identyfikacja metodą SEM cząstek deponowanych na filtrach powietrza zebranych w e współpracy z GIOŚ.