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Surowe dane badawcze do artykułu "Virtually identical does not mean exactly identical: Discrepancy in energy metabolism between glucose and fructose fermentation influences the reproductive potential of yeast cells"
(Archives of Biochemistry and Biophysics, 2024-05-03) Maślanka, Roman; Bednarska, Sabina; Zadrąg-Tęcza, Renata
The physiological efficiency of cells largely depends on the possibility of metabolic adaptations to changing conditions, especially on the availability of nutrients. Central carbon metabolism has an essential role in cellular function. In most cells is based on glucose, which is the primary energy source, provides the carbon skeleton for the biosynthesis of important cell macromolecules, and acts as a signalling molecule. The metabolic flux between pathways of carbon metabolism such as glycolysis, pentose phosphate pathway, and mitochondrial oxidative phosphorylation is dynamically adjusted by specific cellular economics responding to extracellular conditions and intracellular demands. Using Saccharomyces cerevisiae yeast cells and potentially similar fermentable carbon sources i.e. glucose and fructose we analyzed the parameters concerning the metabolic status of the cells and connected with them alteration in cell reproductive potential. Those parameters were related to the specific metabolic network: the hexose uptake - glycolysis and activity of the cAMP/PKA pathway - pentose phosphate pathway and biosynthetic capacities - the oxidative respiration and energy generation. The results showed that yeast cells growing in a fructose medium slightly increased metabolism redirection toward respiratory activity, which decreased pentose phosphate pathway activity and cellular biosynthetic capabilities. These differences between the fermentative metabolism of glucose and fructose, lead to long-term effects, manifested by changes in the maximum reproductive potential of cells.
Stworzenie i walidacja nowego wzoru do obliczania Podstawowej Przemiany Materii u dzieci i młodzieży aktywnych fizycznie - MINIATURA 5
(2023) Łuszczki, Edyta
Measurement or estimation of resting energy expenditure (REE) should be the first step in determining energy demand in physically active boys. The purpose of this study was to develop and validate new equations for resting energy expenditure in male children and adolescents practicing soccer. The cross-sectional studywas carried out among 184 boys in the derivation group and 148 boys in the validation group (mean age 13.20 ± 2.16 years and 13.24 ± 1.75 years, respectively). The calorimeter and device for assessing body composition by bioelectrical impedance analysis (BIA) were used. Model of multiple regression showed that REE can be predicted in this population with Eq. (1) (with height and weight data) or Eq. (2) (with age, height, and fat free mass data). Predictive Eq. (1) had an average error of 51 ± 199 kcal and predictive Eq. (2) − 39 ± 193 kcal. Cohen’s d coefficient was 0.2, which confirms the small difference. The bias was 4.7% and 3.9%, respectively. The accuracy was 61.2% in the population for predictive Eq. (1) and 66.2% for predictive Eq. (2). Therefore, the new equations developed and validated in this study are recommended for the estimation of REE in physically active boys, when the use of IC is not feasible or available.
NGS Bladder cancer
(2024-10-08) Cieśla, Marek
The study group comprises 46 patients with urothelial bladder cancers referred for transurethral resection of the tumour. A molecular study on tumour-derived DNA was performed using next-generation sequencing. Somatic mutations were screened in 50 genes involved in cancerogenesis
Change in patients’ psychophysical performance following lumbar discectomy relative to the postoperative rehabilitation programme
(2019) Wolan-Nieroda, Andżelina; Sobański, Grzegorz; Guzik, Agnieszka; Maciejczak, Andrzej
Abstract
Background. The aim of this study was to assess the change in psychophysical performance of patients after lumbar discectomy in relation to the postoperative rehabilitation programme.
Material and Methods. The study involved 60 participants randomly divided into two groups of 30 individuals. Both groups participated in a basic version of the rehabilitation programme, and individuals in the study group additionally received manual therapy. The evaluation was performed twice, before the start and after the completion of the 3-month rehabilitation programme. The tests were carried out to measure static balance, functional status using Oswestry Disability Index (ODI) and Roland-Morris Disability Questionnaire (RMDQ), lumbar spine range of motion using the original Schober’s test and the intensity of pain using the Visual Analogue Scale (VAS).
Results. Both groups showed significant improvement in most of the psychophysical parameters assessed (study versus control p<0.01;p<0.05), except for the parameters of balance. In the study group, significant changes occurred in all parameters except X average, Area circular and average velocity in trials with eyes closed and left leg stance after rehabilitation (p<0.05). The findings showed significant differences in the reduced pain intensity on the VAS (p=0.0001), improved functional status in ODI and improved static balance (p<0.01), in favour of the study group.
Conclusion. The protocol which additionally included manual therapy was found to be more effective than the basic programme. Its superiority was reflected by greater pain reduction, more visibly improved functional status as well as improved static balance.
Change in patients’ psychophysical performance following lumbar discectomy relative to the postoperative rehabilitation programme
(2019) Wolan-Nieroda, Andżelina; Sobański, Grzegorz; Guzik, Agnieszka; Maciejczak, Andrzej
Abstract
Background. The aim of this study was to assess the change in psychophysical performance of patients after lumbar discectomy in relation to the postoperative rehabilitation programme.
Material and Methods. The study involved 60 participants randomly divided into two groups of 30 individuals. Both groups participated in a basic version of the rehabilitation programme, and individuals in the study group additionally received manual therapy. The evaluation was performed twice, before the start and after the completion of the 3-month rehabilitation programme. The tests were carried out to measure static balance, functional status using Oswestry Disability Index (ODI) and Roland-Morris Disability Questionnaire (RMDQ), lumbar spine range of motion using the original Schober’s test and the intensity of pain using the Visual Analogue Scale (VAS).
Results. Both groups showed significant improvement in most of the psychophysical parameters assessed (study versus control p<0.01;p<0.05), except for the parameters of balance. In the study group, significant changes occurred in all parameters except X average, Area circular and average velocity in trials with eyes closed and left leg stance after rehabilitation (p<0.05). The findings showed significant differences in the reduced pain intensity on the VAS (p=0.0001), improved functional status in ODI and improved static balance (p<0.01), in favour of the study group.
Conclusion. The protocol which additionally included manual therapy was found to be more effective than the basic programme. Its superiority was reflected by greater pain reduction, more visibly improved functional status as well as improved static balance.