Wnuk, MaciejHudecki, AndrzejRzeszutek, IwonaLewińska, AnnaWarski, TymonBaranowska-Korczyc, AnnaWojnarowska-Nowak, RenataBetlej, GabrielaDeręgowska, AnnaHudecki, JacekŁyko-Morawska, DorotaLikus, WirginiaMoskal, AleksandraKrzemiński, PiotrCieślak, MałgorzataKęsik-Brodacka, MałgorzataKolano-Burian, AleksandraWnuk, Maciej2024-03-072024-03-072023-10-01Andrzej Hudecki, Iwona Rzeszutek, Anna Lewińska, Tymon Warski, Anna Baranowska-Korczyc, Renata Wojnarowska-Nowak, Gabriela Betlej, Anna Deręgowska, Jacek Hudecki, Dorota Łyko-Morawska, Wirginia Likus, Aleksandra Moskal, Piotr Krzemiński, Małgorzata Cieślak, Małgorzata Kęsik-Brodacka, Aleksandra Kolano-Burian, Maciej Wnuk, Electrospun fiber-based micro- and nano-system for delivery of high concentrated quercetin to cancer cells, Biomaterials Advances, Volume 153, 2023, 213582, ISSN 2772-9508, https://doi.org/10.1016/j.bioadv.2023.213582.10.1016/j.bioadv.2023.213582https://rdb.ur.edu.pl/handle/item/35word file with experimental data: Figure S1. The gross morphology (top) of polylactide (PLA)-based fibers (PLA), PLA-based fibers containing quercetin (PLA/Q), PLA and polyethylene oxide (PEO)-based fibers (PLA/PEO) and PLA and PEO-based fibers containing quercetin (PLA/PEO/Q) Figure S2. Physical characterization of polylactide (PLA)-based fibers (PLA). Scanning electron microscopy (SEM) was used to analyze the geometry (A, B) and diameter distribution (C) of PLA-based fibers in acetone and chloroform solution; magnification 5000 (A), magnification 10000 (B). (D) XRD diffractogram of PLA-based fibers. (E) Differential thermal analysis/thermogravimetry (DTA/TG) measurements to analyze heat flow (DTA) and mass change (TG) during heating of PLA-based fibers. (F) The analysis of the contact angle of PLA-based fibers using PGX goniometer. Figure S3. Physical characterization of PLA-based fibers containing quercetin (PLA/Q). Scanning electron microscopy (SEM) was used to analyze the geometry (A, B) and diameter distribution (C) of PLA/Q in acetone and chloroform solution; magnification 5000 (A), magnification 10000 (B). (D) XRD diffractogram of PLA/Q. (E) Differential thermal analysis/thermogravimetry (DTA/TG) measurements to analyze heat flow (DTA) and mass change (TG) during heating of PLA/Q. (F) The analysis of the contact angle of PLA/Q using PGX goniometer. Figure S4. Physical characterization of PLA and polyethylene oxide (PEO)-based fibers (PLA/PEO). Scanning electron microscopy (SEM) was used to analyze the geometry (A, B) and diameter distribution (C) of PLA/PEO in acetone and chloroform solution; magnification 5000 (A), magnification 10000 (B). (D) XRD diffractogram of PLA/PEO. (E) Differential thermal analysis/thermogravimetry (DTA/TG) measurements to analyze heat flow (DTA) and mass change (TG) during heating of PLA/PEO. (F) The analysis of the contact angle of PLA/PEO using PGX goniometer. Figure S5A. AFM-based characterization of PLA-based fibers (PLA). Figure S5B. AFM-based characterization of PLA-based fibers containing quercetin (PLA/Q). Figure S5C. AFM-based characterization of PLA- and PEO-based fibers (PLA/PEO). Figure S5D. AFM-based characterization of PLA- and PEO-based fibers containing quercetin (PLA/PEO/Q). Figure S5E. AFM-based analysis of the roughness of PLA, PLA/Q, PLA/PEO and PLA/PEO/Q fibers. Figure S6. XRD diffractogram of free quercetin (A) and differential thermal analysis/thermogravimetry (DTA/TG) measurements to analyze heat flow (DTA) and mass change (TG) during heating of free quercetin (B). Figure S7. The Raman spectra of PLA (A), PLA/Q (B), PLA/PEO (C), and PLA/PEO/Q (D). Figure S8. (A) Calibration curve used for the evaluation of quercetin concentrations in aqueous solution upon the incubation of PLA/Q and PLA/PEO/Q in cell-free in vitro systems (Fig. 2A). Figure S8 (B) Representative Dot plots used in apoptosis analysis in osteosarcoma cells (MG-63, U-2 OS and SaOS-2 cells) - Figure S8 (C) Representative Histograms used in analysis in cell cycle progression in osteosarcoma cells (MG-63, U-2 OS and SaOS-2 cells) Figure S9A. Representative Histograms used to oxidative stress analysis (A), Akt and ERK1/2 activation in osteosarcoma cells Figure S9B. Representative dot-plots used to analysis of Akt and ERK1/2 activation in osteosarcoma cells Figure S9C immunofluorescence changes in the levels of HSP70 and HSP90 in osteosarcoma cells Figure S10A. Representative Dot plots used in apoptosis analysis in breast cancer cells Figure S10B. The analysis of fiber hemocompatibility Exel file with dataset used to prepare the chartsThe anticancer potential of quercetin (Q), a plant-derived flavonoid, and underlining molecular mechanisms are widely documented in cellular models in vitro. However, biomedical applications of Q are limited due to its low bioavailability and hydrophilicity. In the present study, the electrospinning approach was used to obtain polylactide (PLA) and PLA and polyethylene oxide (PEO)-based micro- and nanofibers containing Q, namely PLA/Q and PLA/PEO/Q, respectively, in a form of non-woven fabrics. The structure and physico-chemical properties of Q-loaded fibers were characterized by scanning electron and atomic force microscopy (SEM and AFM), X-ray powder diffraction (XRD), differential scanning calorimetry (DSC), goniometry and FTIR and Raman spectroscopy. The anticancer action of PLA/Q and PLA/PEO/Q was revealed using two types of cancer and nine cell lines, namely osteosarcoma (MG-63, U-2 OS, SaOS-2 cells) and breast cancer (SK-BR-3, MCF-7, MDA-MB-231, MDA-MB-468, Hs 578T, and BT-20 cells). The anticancer activity of Q-loaded fibers was more pronounced than the action of free Q. PLA/Q and PLA/PEO/Q promoted cell cycle arrest, oxidative stress and apoptotic cell death that was not overcome by heat shock protein (HSP)-mediated adaptive response. PLA/Q and PLA/PEO/Q were biocompatible and safe, as judged by in vitro testing using normal fibroblasts. We postulate that PLA/Q and PLA/PEO/Q with Q releasing activity can be considered as a novel and more efficient micro- and nano-system to deliver Q and eliminate phenotypically different cancer cells.enAttribution 4.0 InternationalApoptosisBreast cancerElectrospun fibersHSPOsteosarcomaQuercetinprocessed dataset